BACT/ALERT® 3D Microbial Detection Systems

BACT/ALERT® 3D Microbial Detection Systems

Get innovation in septicemia diagnosis for any size laboratory with the BACT/ALERT® 3D automated microbial detection systems

  • No compromise: performance, flexibility, workflow & safety
  • Intuitive, easy-to-use system requires little training
  • Recovery of a wide range of organisms
  • Unique shatter-resistant plastic bottles maximize safety


The BACT/ALERT® 3D family of products brings you sophisticated automated microbial detection systems with advantages in every dimension of blood culture testing from safety and design to recovery and accuracy. They provide optimal environments for the recovery of a wide range of microorganisms, including bacteria, fungi and mycobacteria. All this in a single innovative platform that’s the most compact, modular and flexible system available.

Flexible & scalable to suit any lab

The BACT/ALERT® system is a flexible and scalable family of three instruments with various capacities – so they offer advantages to suit different sized labs with different needs.

  • Add additional testing capacity as you need it, thanks to expandable design
  • Small footprint – stackable units fit any laboratory layout
  • Choose number of incubator modules for your lab’s annual bottle testing volume
  • Configure each incubator module to perform blood culture or body fluid, platelet sterility testing, and/or mycobacteria testing, by drawer

BACT/ALERT® 3D 240: Capacity and capability combined

  • Four incubator drawers of 60 cells deliver 240 cell capacity
  • Control module handles up to 6 incubator modules for capacity up to 1440 cells
  • Horizontal and vertical modularity minimize physical strain
  • Sample-loading flexibility with left- or right-handed sample loading drawers
  • Drawers configured for blood or mycobacterium cultures


BACT/ALERT® 3D 120 Combo: Surprisingly compact, remarkably complete

  • Combines control and incubator modules into a single instrument
  • 120 cell capacity in two drawers
  • Connect to up to 3 incubator modules to one Combo instrument
  • Sample-loading flexibility with left- or right-handed sample loading drawers
  • Drawers configured for blood or mycobacterium cultures


BACT/ALERT® 3D 60: Simply smaller

  • 60 cell capacity (3,600 blood/body fluids annually)
  • Optimized space saving & cost-effective design – so any laboratory can benefit from BACT/ALERT® 3D technology
  • Configurable for blood or mycobacterium cultures

Workflow that’s as easy as 1-2-3

Sometimes, the simplest things make the biggest difference. As labs face the challenges of higher volumes, demand for reduced turnaround and the need to use staff resources effectively, smart workflow is critical. The efficient workflow you get with BACT/ALERT® 3D instrument helps you meet those challenges, reduce risk of error and gain peace-of-mind through easy and intuitive bottle handling. In fact, the bottle-handling process is as easy as 1-2-3 with the three-step bottle loading process of Touch, Scan, Load:

  • Exclusive touch-screen operation enables text-free control, facilitates bottle handling and provides instant visual and audio alerts for positive bottles
  • Bar-code scanner reads bottle ID and LIS accession numbers for faster, easier bottle entry
  • Patented cell flags in each cell instantly recognize bottle movements and let you load bottles anywhere in the system
  • Easy procedure minimizes training requirements and enhances productivity

Safe and reliable media

The BACT/ALERT® system uses media cleared for use with blood, sterile body fluids and platelets, as well as specialized media for detection of mycobacteria. Unique colorimetric technology outshines other technology by reducing false negatives due to delayed bottle entry. Triple-layer bottle design ensures appropriate environment for reliable recovery of microorganisms.

  • Safety and efficiency are ensured with plastic blood culture media, pioneered by bioMérieux
  • Shatter-resistant bottles reduce biohazard exposure risk
  • Light-weight bottles offer cost-effective shipping and disposal – without compromising results


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Technical specifications and reference numbers


Control Module Specifications
Height36 inches90 cm
Width14 inches35 cm
Length24.3 inches60.8 cm
Unload Weight91 lbs41.3 kg
Electric Power Service Requirements100/120volts (50-60Hz)110/240v
Power Consumed in Watts115 VAC 72 Watts typical
Heat Dissipated245 BTU/Hr. 2840 maximum
Sound Emission46.4 dB


Incubator Module (240 cell capacity)
Height36 inches90 cm
Width19.5 inches48.8 cm
Length24.3 inches60.8 cm
Unload Weight200 lbs90.8 kg
Load Weight233 lbs105.8 kg
Capacity240 cells (60 cells / drawer)
Electric Power Service Requirements100/120 volts (50-60Hz)110/240v
Power Consumed in Watts115 VAC 256 Watts (idle), 640 Watts typical
Heat Dissipated904 BTU/Hr. 2840 maximum
Sound Emission55.5 dB

243010 BacT/ALERT® Controller Module

210159 BacT/ALERT® Incubator Module Left w/ Racks

210161 BacT/ALERT® Incubator Module Right w/ Racks

BACT/ALERT® 3D 120 Combo

Height30.8 inches77 cm
Width19.5 inches48.8 cm
Length24.5 inches61.2 cm
Unload Weight200 lbs90.8 kg
Load Weight216.5 lbs98.3 kg
Capacity120 cells (60 cells/drawer)
Electric Power Service Requirements100/120 VAC (50-60Hz)110/240v
Power Consumed in Watts115 VAC 256 Watts (idle), 640 watts typical
Heat Dissipated904 BTU/Hr. 2840 maximum
Sound Emission55.5 dB

247001 BACT/ALERT® 120 Combination Module Right
247004 BACT/ALERT® 120 Combination Module Left


Height24 inches60 cm
Width23.1 inches57.8 cm
Length19.5 inches48.8 cm
Unload Weight90 lbs40.9 kg
Load Weight98 lbs44.5 kg
Capacity60 cells
Electric Power Service Requirements100/120 VAC (50-60Hz)110/240v
Power Consumed in Watts115 VAC 72 Watts typical
Heat Dissipated245 BTU/Hr. 2840 maximum
Sound Emission50 dB

248001 BacT/ALERT® 3D 60

Data Management Options

BACT/ALERT® 3D Select software provides basic data management functions. Five customizable data fields can be used to add patient demographics or culture information. Reports can be generated based upon data entered into customizable fields or instrument-generated data. Reports are customizable with sorting and printing capabilities.

BACT/ALERT® 3D SelectLink software offers the same capabilities as BACT/ALERT® 3D Select but in addition has an interface to the Laboratory Information System (LIS) to eliminate manual data entry. This option is perfect for laboratories that rely on their LIS for most of their reporting needs.

BACT/ALERT® 3D Signature software includes OBSERVA®, a comprehensive, flexible and customizable data management system. Signature is an ideal choice for demanding environments where site-specific operating modes and customized data manipulation and reporting are necessities. It offers user-defined data fields with options lists, configurable icons to launch functions or print reports, numerous pre-programmed reports which are also customizable, and the ability to query the database by virtually any parameter. The BACT/ALERT® 3D Signature system includes an interface from BACT/ALERT® 3D to the LIS.


Standard Media Recovery:

Mirret S, Reller LB, Petti CA, Woods CW, Vazirani B, Sivadas R, and Weinstein MP. Controlled Clinical Comparison of BacT/ALERT® Standard Aerobic Medium with BACTEC Standard Aerobic Medium for Culturing Blood. J. Clin Microbiol. 2003; 41(6):2391-2394.

Bernard D, Verschraegen G, Claeys G, Lauwers S, Rosseel P. Bilophila wadsworthia bacteremia in a patient with gangrenous appendicitis. Clin Infect Dis. June 1994;18:1023-1024.

Standard vs. FAN® Media:

Weinstein MP, Mirrett S, Reimer LG, Wilson ML, Smith-Elekes S, Chuard CR, Joho KL, Reller LB. Controlled evaluation of BacT/ALERT standard aerobic and FAN® aerobic blood culture bottles for detection of bacteremia and fungemia. J Clin Microbiol. 1995;33(4):978-981.

Wilson ML, Weinstein MP, Mirrett S, Reimer LG, Feldman RJ, Chuard CR, Reller LB. Controlled evaluation of BacT/ALERT® standard anaerobic and FAN® anaerobic blood culture bottles for the detection of bacteremia and fungemia. J Clin Microbiol. 1995;33(9):2265-2270.

McDonald LC, Fune J, Gaido LB, Weinstein MP, Reimer LG, Flynn TM, Wilson ML, Mirrett S, Reller LB. Clinical importance of increased sensitivity of BacT/ALERT® FAN® aerobic and anaerobic blood culture bottles. J Clin Microbiol. 1996;34(9):2180-2184.

Mirrett S, Everts RJ, Reller LB. Controlled Comparison of Original Vented Aerobic FAN® Medium with New Nonvented BacT/ALERT® FA Medium for Culturing Blood. J Clin Microbiol. 2001;39(6):2098-2101.

Mirrett S, Petti CA, Woods CW, Magadia R, Weinstein MP, Reller LB. Controlled Clinical Comparison of the BacT/ALERT® FN and the Standard Anaerobic SN Blood Culture Medium. J Clin Microbiol. 2004;42(10):4581-4585.

 FAN® vs. BACTEC Plus:

Ziegler R, Johnscher I, Martus P, Lenhardt D, Just HM. Controlled clinical laboratory comparison of two supplemented aerobic and anaerobic media in automated blood culture systems to detect bloodstream infections. J Clin Microbiol. 1998; 36(3):657-661.

Pohlman JK, Kirkley BA, Easley KA, Basille BA, Washington JA. Controlled clinical evaluation of BACTEC Plus Aerobic/F and BacT/ALERT® aerobic FAN® bottles for detection of bloodstream infections. J Clin Microbiol. 1995;33(11):2856-2858.

Jorgensen JH, Mirrett S, McDonald LC, Murray PR, Weinstein MP, Fune J, Trippy CW, Masterson M, Reller LB. Controlled clinical laboratory comparison of BACTEC Plus Aerobic/F Resin medium with BacT/ALERT® Aerobic FAN® medium for detection of bacteremia and fungemia. J Clin Microbiol. 1997;35(1):53-58.

FAN Plus Media:

Altun O, Almuhayawi M, Ullberg M and Ozenci V, Clinical Evaluation of the FilmArray Blood Culture Identification Panel in Identification of Bacteria and Yeasts from Positive Blood Culture Bottles, J. Clin. Microbiol. 2013, 51(12):4130.

Amarsy-Guerle R, Mougari F, Jacquier H, Oliary J, Benmansour H, Riahi J, Bercot B, Raskine L and Cambau E. High medical impact of implementing the new polymeric bead-based BacT/ALERT® FA Plus and FN Plus blood culture bottles in standard care, Eur J Clin Microbiol Infect Dis. 2015 Feb. Doi: 10.1007/s10096-015-2319-8

Doern C, Mirrett S, Halstead D, Abid J, Okada P and Reller B. Controlled Clinical Comparison of New Pediatric Medium with Adsorbent Polymeric Beads (PF Plus) versus Charcoal-Containing PF Medium in the BacT/Alert Blood Culture System.  J. Clin. Microbiol. 2014 52(6): 1898-1900.

Kirn TJ, Mirrett S, Reller LB, Weinstein MP, Controlled Clinical Comparison of BacT/ALERT FA Plus and FN Plus Blood Culture Media with BacT/ALERT FA and FN Blood Culture Media, J. Clin. Microbiol. doi:10.1128/JCM.03063-13.

Lee DH, Kim SC, Bae IG, Kim S, Clinical Evaluation of BacT/Alert FA Plus and FN Plus Bottles Compared with Standard Bottles, J. Clin. Microbiol. 2013, 51(12):4150.

Mitteregger D, Barousch W, Nehr M, Kundi M, Zeitlinger M , Makristathis A and Hirschl AM, Neutralization of Antimicrobial Substances in New BacT/Alert FA and FN Plus Blood Culture Bottles, J. Clin. Microbiol. 2013, 51(5):1534.

FAN Plus Media vs. BACTEC Plus:

Fiori B, D’Inzeo T, Di Florio V, De Maio F, De Angelis G, Giaquinto A, Campana L, Tanzarella E, Tumbarello M, Antonelli M, Sanguinetti M, Spanu T. Performance of Two Resin-Containing Blood Culture Media in Detection of Bloodstream Infections and in Direct Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry (MALDI-TOF MS) Broth Assays for Isolate Identification: Clinical Comparison of the BacT/Alert Plus and Bactec Plus Systems.  J. Clin. Microbiol.  2014 52(10): 3558-3567

Body Fluids:

Bourbeau P, Riley J, Heiter BJ, Master R, Young C, Pierson C. Use of the BacT/ALERT® blood culture system for culture of sterile body fluids other than blood. J Clin Microbiol. 1998;36(11):3273-3277.

Alfa MJ, Degagne P, Olson N, Harding KM. Improved detection of bacterial growth in continuous ambulatory peritoneal dialysis effluent by use of BacT/ALERT® FAN® bottles. J Clin Microbiol. 1997;35(4):862-866.

Glass vs. Plastic Bottles:

Snyder JW, Munier GK, Bostic GD, Bozigar PS, Hanna R. Evaluation of a Plastic Nonvented Aerobic Blood Culture bottle for Use with the BacT/ALERT® Microbial Detection System. J Clin Microbiol 2002; 40(12): 4757-4759.

Petti, CA, Mirrett S, Woods CW, and Reller LB. Controlled Clinical Comparison of Plastic versus Glass Bottles of BacT/ALERT®PF Medium for Culturing Blood from Children. J Clin Microbiol 2005; 43(1): 445–447

Petti CA, Mirrett S, Woods CW, Reller LB, Controlled clinical comparison of plastic and glass bottles of BacT/ALERT® FA medium for culturing organisms from blood of adult patients. J Clin Microbiol 2005; 43(4), 1960-1962


Alpern E, Alessandrini E, Bell L, Shaw K, McGowan L. Occult bacteremia from a pediatric emergency department: current prevalence, time to detection, and outcome. Pediatrics. 2000;106(3):505-511.

McGowan K, Foster J, Coffin S. Outpatient pediatric blood cultures: time to positivity. Pediatrics. 2000;106(2):251-255.

Krishner KK, Gibb P, CorbettS, and Church DEIDRE. Comparison of the BacT/ALERT® PF Pediatric FAN® Blood Culture Bottle with the Standard Pediatric Blood Culture Bottle, the Pedi-BacT J Clin Microbiol. 2001;39(8): 2880-2883.


Welby-Sellenriek PL, Keller DS, Ferrett RJ, Storch GA. Comparison of the BacT/ALERT® FAN® aerobic and the Difco ESP 80A aerobic bottles for pediatric blood cultures. J Clin Microbiol. 1997;35(5):1166-1171.

Doern G, Barton A, Rao S. Controlled comparative evaluation of BacT/ALERT® FAN® and ESP 80A aerobic media as means for detecting bacteremia and fungemia. J Clin Microbiol. 1998; 35(9):2686-2689.

Mycobacteria, Yeast and Fungi:

Alcaide F, Benitez MA, Escriba JM, Martin R. Evaluation of the BACTEC MGIT™ 960 and the MB/BacT systems for recovery of mycobacteria from clinical specimens and for species identification by DNA AccuProbe. J Clin Microbiol. 2000;38(1):398-401.

Crump J, Tanner DC, Mirrett S, McKnight CM, Reller LB, Controlled Comparison of BACTEC 13A, Myco/F Lytic, BacT/ALERT® MB and Isolator 10 Systems for Detection of Mycobacteremia. J Clin Microbiol 2003;41(5):1987-1990.

McDonald, L., Weinstein, M., Fune, J., Mirrett, S., Reimer, L., Reller, B., Controlled Comparison of BacT/ALERT® FAN® Aerobic Medium and Bactec Fungal Blood Culture Medium for Detection of Fungemia, J Clin Microbiol 2001:39(2): 622–624.


Bourbeau P and Foltzer M. Routine Incubation of BacT/ALERT FA and  FN Blood Culture Bottles for More than 3 Days May Not Be Necessary.  J Clin Microbiol. 2005: 43(5): 2506-2509.

Seegmuller I, Eschenbach U, Kamereck K. Sensitivity of the BacT/ALERT® FA medium for detection of Pseudomonas aeruginosa in pre-incubated blood cultures and its temperature dependence. J Med Microbiol, (2004); 53. 869-874.

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